Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Hach Company pPb-1 Acid Preservative Solution, 236 mL
For Lead determination by the LeadTrak™ Fast Column Extraction method. Hach method 8317.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline powder, pH 7.4, for preparing 5 L solutions | MFCD00131855 | 10/pk
Phosphate buffered saline powder, pH 7.4, for preparing 5 L solutions | | MFCD00131855 | 10/pk
Biotium Fixation Permeabilization Other Buffers Buffers Accessories and Background Reducers Fixation Permeabilization and Other Buffers Chemical Reagents 50/PK
PBS (phosphate-buffered saline) buffer powder packet for preparing 2 L of 1X PBS buffer Box includes 50 packets
![Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 980g make, 10 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661556.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 980g make, 10 L
PBS [Phosphate Buffered Saline] 10X Powder Concentrate, in a 980g pack, makes 10 liters of 10X PBS solution. This highly concentrated powder is perfect for laboratories that require large volumes of PBS for a wide range of biological and biochemical applications. The powder dissolves easily in water, providing a quick and convenient way to prepare the solution. 10X PBS is essential in many laboratory protocols, including protein assays, cell culture, and washing procedures. The larger pack size is ideal for high-throughput laboratories or research facilities that need to prepare PBS solutions in bulk for routine use in experiments and testing.
Electron Microscopy Sciences Phosphate Buffer 0.1M, pH 6.0 1 LT
Prepared fresh for each order from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for biological research only. Not to be used for medical or any other purposes.
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 2.0 100 ML
PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg (100 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Apexbio Technology LLC Annexin V-HF488/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes Propidium iodide (PI) selectively binds double-stranded DNA and yields a red fluorescence PI is membrane-impermeable in healthy cells but can penetrate the membranes of late apoptotic and dead cells staining their nuclei Simultaneous staining with Annexin V-HF488 and PI enables differentiation between early apoptotic late apoptotic and dead cells
Biotang Inc Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S
Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S.
Apexbio Technology LLC Protease Inhibitor Cocktail (100X in DMSO EDTA plus) 100x(1ml A 1ml B)
This product is a protease inhibitor cocktail (100X in DMSO EDTA plus) specifically designed for use during cell lysis and protein extraction processes It is a ready-to-use concentrated solution that can inhibit a variety of endogenous proteases Also included is a separate 0 5 M EDTA solution which effectively inhibits metalloproteases The inhibitor cocktail consists of six carefully optimized broad-spectrum protease inhibitors dissolved in DMSO capable of efficiently inhibiting serine proteases cysteine proteases aspartic proteases and aminopeptidases commonly present in cell lysates EDTA is provided for inhibition of metalloproteases If immobilized metal affinity chromatography (IMAC) is involved for protein purification or if two-dimensional (2D) gel electrophoresis analysis is planned EDTA must be thoroughly removed by extensive dialysis or desalting prior to purification Specification 1 Set equals 1X (1 mL A 1 mL B) 10 Sets equals 10X (1 mL A 1 mL B) and so on
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 0.1 6X500ML
PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg (6 x 500 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 0.1% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
ILEX LIFE SCIENCES LLC 2-MERCAPTOETHANOL 50MM 100ML
PAN-Biotech 2-Mercaptoethanol 50 mM in DPBS (100 ml). DESCRIPTION: 2-Mercaptoethanol, also known as beta-mercaptoethanol (BME), is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, thus protocols generally require daily supplementation. PAN-Biotech 2-Mercaptoethanol is supplied at a concentration of 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). SOLUTION: 50 mM in DPBS. STORAGE: +2-8°C. STERILE: Yes. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Teknova 1X PBS-TBN Buffer with 0.05% Tween, 1% BSA, 0.1% Sodium Azide. 1000mL, Sterile. 4 pack.
150mM Sodium Chloride, 50mM Monosodium Phosphate,1.00% Bovine Serum Albumin, 0.10% Sodium Azide,0.05% Tween-20 (v/v)
Sigma Aldrich Fine Chemicals Biosciences Dulbecco’s Phosphate Buffered Saline, 1000 mL
Dulbecco’s Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process, where the presence of calcium and magnesium can inhibit trypsin activity. For this application, use DPBS Modified, which is formulated without calcium and magnesium salts.
Bio Rad Laboratories 1X PHOSPHATE BUFFERED SALINE W
Blocking Reagent; 1X Phosphate Buffered Saline With 1% Casein; Bio-Rad; 10mM sodium phosphate, 150um NaCl, 1% (w/v) casein, pH 7.4; 1L