Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt 99.5 tit1KG
HEPES sodium salt 99.5 tit1KG
Hampton Research HR2-785/1.0 M HEPES pH 7.0 - 100 ml
HR2-785/1.0 M HEPES pH 7.0 - 100 ml. Please note items from this supplier are non-returnable.
Boston Bioproducts Inc MES Buffer (1 M, pH 5.0) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Research Products International Corp Bicine Ultra-Pure, 100 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Alkali Scientific CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL
CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-50.03.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 50 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified | 7365-45-9 | MFCD00006158 | 25g
HEPES BioPerformance Certified | 99.5% | 238.3 | 7365-45-9 | MFCD00006158 | 25g
Sigma Aldrich Fine Chemicals Biosciences MES hydrate | 1266615-59-1 | MFCD00149409 | 100 g
MES hydrate | Purity: 99.5% | Mol Wt: 195.24 | 1266615-59-1 | MFCD00149409 | 100 g
![Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40020-500.0.JPG-250.jpg)
Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 500 Grams
CHES, also known as 2-(N-Cyclohexylamino) ethanesulfonic acid, is a chemical compound that plays a significant role in life science research, particularly in molecular biology and protein research.
CHES is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 8.6 to 10.0, making it suitable for applications requiring higher alkaline pH conditions.
CHES is also commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability in alkaline conditions make it valuable in these applications.
One of the significant advantages of CHES is its low ionic strength, which minimizes interference with biological processes and enzymatic activities.
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 25 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier.
- Buffering agent with a useful pH range from 7.3 to 8.7.
- Reduces Aβ-aggregate-induced memory deficits.
- Rescues cognitive deficits in mice.
- Orally active.
- Penetrates the blood-brain barrier.
Electron Microscopy Sciences Hepes, Crystal, Biological Buffer, 7.0-8.0 pH 100 GR
(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
CAS #7365-45-9 C₈H₁₈O₄N₂S F.W. 238.30
Assay: >99%
Good's buffer.
pH: 7.0-8.0