Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
PLANT CELL TECHNOLOGY 1X Phosphate Buffered Saline Solution, pH 7.4, without Calcium and Magnesium, Buffer Solution, Mod. 6, Clear Liquid, Sterile, 500mL bottle.
1X PBS Solution Mod. 6, pH 7.4, is a sterile buffer used in mammalian cell culture to maintain physiological pH during washing, dilution, or reagent preparation. Packaged in a clear 500mL bottle. Ready-to-use and stable under recommended conditions. Ideal for research labs, tissue culture, and molecular biology workflows.
Biotang Inc Phosphate-Buffered Saline PBS, 10X, pH 7.4, 1000 ml
Phosphate-Buffered Saline PBS, 10X, pH 7.4, 1000 ml.
CAPTIVATE BIO LLC DPBS no Cal. or Mag.
Captivate Bio s DPBS is a balanced salt solution used for various cell culture applications like cell washing transporting cells and diluting cells for counting This formulation is configured without calcium or magnesium Manufactured in the US under ISO and GMP conditions 12 x 1000mL
United States Biological Corporation DULBECCO MEM DMEM PWDR D980028
NC3314668 DULBECCO MEM DMEM PWDR D980028
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
DULBECCOS Dulbeccos Phosphate Bufer500ML
ILEX LIFE SCIENCES LLC TRYPSIN P10-021500 6 X 500ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (6 x 500 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Quality Biological Inc PBS (10X), pH 7.4, 10 Liters
Balanced salt solution ¿ Wash cells before dissociation ¿ Transport cells or tissue ¿ Dilute cells for counting ¿ Prepare reagents ¿ Endotoxin and Mycoplasma tested ¿ Useful in immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Osmolality: 300 ¿ 55 mOsm/Kg H20 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿Max Shelf Life: 24 months
Kd Medical Inc PBS BUFFER, 1X, PH 7.2
Standard buffer for many biomolecular procedures. One 1 Liter bottle.
FUJIFILM BIOSCIENCES INC MEM 1X Earles Salts w/o NEAA
MEM 1X Earles Salts w/o NEAA is an uncomplicated medium that is useful for a wide range of species and cells
Ethos Biosciences Dilurex 10X PBS Buffer, 100ml, Ethos
Dilurex 10X PBS is a concentrated solution of phosphate buffered saline intended as a stock solution. Save space by keeping 10x concentrate and dilute to 1x pH 7.4 working concentration when needed by mixing 1 part 10X PBS with 9 parts distilled water. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 10X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
![Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0mL Pre Filled Tubes, Sterile, 1000 Tubes per Case, 2.0mL Tube](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661558.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 1.0mL Pre Filled Tubes, Sterile, 1000 Tubes per Case, 2.0mL Tube
PBS (Phosphate Buffered Saline) 1X Solution is offered in a large, ready-to-use format with 1.0mL pre-filled tubes, making it suitable for laboratory applications that require rapid, high-volume processing. With 1000 tubes per case, this product is ideal for clinical laboratories, research facilities, and biotechnological applications that perform large-scale sample analysis. PBS helps maintain osmotic pressure and pH stability, which is essential for preserving cell integrity during experiments. The pre-filled tubes offer convenience and reduce contamination risks, making them ideal for use in microbiology, molecular biology, and cell culture studies where consistent buffer conditions are required.
Growcells PBS 10X 1L CS12
Sterile 10X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 80g, KCl 2g, Na2HPO4 14.4g, KH2PO4 2.4g