Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Ethos Biosciences Dilurex 1X PBS Buffer, 1000ml, Ethos
Dilurex 1X PBS is a ready-to-use working concentration of pH 7.4 phosphate buffered saline. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 1X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
Medchemexpress LLC N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid | 10191-18-1 | 98.0% | 500 G
BES is a secondary standard biochemical buffer used in diagnostic testing. It offers a stable pH environment for various applications.
- Effective pH range of 6.4 to 7.8
- pKa of 7.15 at 20 °C
- Suitable for diagnostic testing areas
Medchemexpress LLC Mops | 1132-61-2 | 99.95% | 25 G
MOPS is a buffering agent used in biology. It is known for its ability to maintain the pH of mammalian cell culture media and significantly improve the thermal stability of BSA.
- Buffering agent for biological applications.
- Maintains pH of mammalian cell culture media.
- Improves thermal stability of BSA.
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 48 Grams of Powder, Makes 10 Liters of Solution
Murashige & Skoog Medium with MES Buffer is a plant tissue culture medium designed for larger-scale applications. The 10-liter solution, made from 48 grams of powder, is enhanced with MES buffer, which provides pH stability during plant cell culture. This medium is essential in plant biotechnology studies where pH control is crucial for ensuring the proper growth and development of plant cells. The MES buffer helps prevent pH fluctuations that could disrupt cell metabolism and growth. This formulation is widely used in laboratories involved in plant tissue culture, regeneration, and studies of plant cell development, offering a reliable solution for maintaining consistent pH levels.
Bioworld ADA Buffer, 0.2M, pH 6.0, 100 mL
Product DescriptionADA (N-(2-acetamido)iminodiacetic acid) buffer has a concentration of 0.2M and a pH value of 6.0. It has a molecular weight of 190.2 and formula of C6H10N2O5. ADA is a zwitterionic buffer used in biochemistry and molecular biology, such as its use in protein-free medium for supporting chick embryo fibroblasts. It has also been shown to interfere with color development in bicinchoninic acid assay (BCA) for protein quantitation and used in isoelectric focusing of proteins in immobilized pH gradients.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
![Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659974.jpg-250.jpg)
Alkali Scientific MOPS [3 (N morpholino) propanesulfonic acid], 1 Kg
MOPS (3-(N-morpholino)propane-1-sulfonic acid) is a commonly used biochemical reagent for maintaining stable pH conditions in laboratory applications. This 1kg package is ideal for preparing large quantities of buffer solutions used in biological and biochemical experiments. MOPS buffers are effective in the pH range of 6.5 to 7.9, making them suitable for enzyme reactions, protein studies, and DNA/RNA work. It is widely used in electrophoresis, chromatography, and molecular biology assays where consistent pH levels are crucial. This reagent is essential in maintaining the stability of biological macromolecules, ensuring reproducibility in experimental results.
Hampton Research 100% TACSIMATE PH 8.0 200ML
HR2-829/100% Tacsimate pH 8.0 - 200 ml. Please note items from this supplier are non-returnable.
![Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660391.jpg-250.jpg)
Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G
DIPSO (3 [N,N-Bis(2 Hydroxyethyl)amino] 2-hydroxypropane sulfonic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is widely used in molecular biology, biochemistry, and analytical chemistry for maintaining stable pH levels in low pH environments. DIPSO is ideal for enzyme assays, protein research, and chromatography, where maintaining a precise pH is critical. It is also useful in protein purification and electrophoresis, providing consistent and reliable results. The 25g size is perfect for smaller scale experiments or routine laboratory applications where a high-quality buffering solution is necessary for pH regulation.
FUJIFILM Biosciences RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659887.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 500 Grams
PIPES Sodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt) is a biochemical reagent used to create buffer solutions that maintain a stable pH environment. This 500g quantity is ideal for large-scale laboratory applications, such as enzyme reactions, protein chemistry, and molecular biology experiments. PIPES sodium salt buffers maintain a stable pH around 7.0, which is essential for preserving enzyme activity and protein integrity in biological assays. The high buffering capacity of PIPES sodium salt ensures pH consistency, making it a popular choice in cellular studies, microbiology, and biochemical research involving delicate biochemical reactions.
eMolecules MES Monohydrate | 145224-94-8 | MFCD00149409 | 100g
Chem-Impex | MES Monohydrate | 100g | 288406858 | 21885 | | 145224-94-8 | MFCD00149409 | 213.250 | C6H15NO5S
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Hampton Research HR2-817/1.0 M ADA pH 6.5 - 100 ml
HR2-817/1.0 M ADA pH 6.5 - 100 ml. Please note items from this supplier are non-returnable.